Shahed UniversityImmunoregulation2588_52008120250101An Index to Assess Integrative Health Status in Human Studies498910.32598/Immunoregulation.8.18ENMohammad-RezaVaez-MahdaviHealth Equity Research Center, Shahed University, Tehran, Iran.0000-0002-1169-1703LeilaNasiriDepartment of Health Equity, Immunoregulation Research Center, Shahed University, Tehran, IranJournal Article20251115<strong>Background</strong>: Conventional metrics for assessing biological aging, like allostatic load or telomere length, have limitations in providing a comprehensive picture, especially across diverse populations exposed to chronic stressors like sulfur mustard.<br /><strong>Materials and Methods</strong>: This study proposes a novel composite index the Biological Health Score (BHS)-to-telomere length ratio. It integrates a multisystem BHS (from non-invasive biomarkers) with leukocyte telomere length, applicable to various epidemiological studies.<br /><strong>Results</strong>: The composite ratio demonstrated greater explanatory power than its individual components. It more successfully predicted injury severity in sulfur mustard-exposed veterans and showed a stronger association with biological aging markers.<br /><strong>Conclusion</strong>: This feasible, integrative index is a promising tool for epidemiological and clinical research to identify individuals at risk of accelerated aging and health disorders resulting from chronic stress exposure.https://immunoreg.shahed.ac.ir/article_4989_702ebec69fb8a188478c788aa1df5fa6.pdfShahed UniversityImmunoregulation2588_52008120250101The First Global Report of Vindolinine in Vinca herbacea From the Hyrcanian Floristic Region: GC–MS Identification and Potential Anticancer Relevance499010.32598/Immunoregulation.8.19ENMohammadKordkatouliDepartment of Genetics, TeMS. C, Islamic Azad University, Tehran, Iran.0009-0006-3008-6820Mehr AliMahmood JanlouMedicinal Plants Research Center, Go. C, Islamic Azad University, Gorgan, Iran.
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Department of Cell and Molecular Biology, Go. C, Islamic Azad University, Gorgan, Iran.AliVarasteh MoradiMedicinal Plants Research Center, Go. C, Islamic Azad University, Gorgan, Iran.
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Department of Chemistry, Go. C, Islamic Azad University, Gorgan, Iran.MuhammadRizwanDepartment of Bioinformatics and Biological Statistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.AryanSateeiMedicinal Plants Research Center, Go. C, Islamic Azad University, Gorgan, Iran.
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Department of Plant Science, Go. C, Islamic Azad University, Gorgan, Iran.0000-0001-6810-6790Mohammad AminJavidiDepartment of Integrative Oncology, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.Journal Article20251211<strong>Background</strong>: Vinca herbacea Waldst. & Kit. is a relatively understudied species of the Apocynaceae family, distributed across temperate regions of Asia and Europe, with verified populations in northern Iran, particularly within the Hyrcanian forests. The family is well known for producing monoterpenoid indole alkaloids (MIAs), such as vinblastine and vincristine, which possess potent anticancer activities. Vindolinine, an important indole alkaloid and a biosynthetic precursor of these chemotherapeutic agents, has not been previously reported in V. herbacea.<br /><strong>Materials and Methods: </strong>In June 2024, aerial parts of V. herbacea (leaves, stems, and flowers) were collected from the Baleskuh Protected Area in Mazandaran Province, Iran. Plant materials were extracted using ethanol, n-propanol, and butanol through cold maceration, and all extracts were analyzed using gas chromatography–mass spectrometry (GC–MS). Compound identification was performed using National Institute of Standards and Technology (NIST) and Wiley spectral libraries.<br /><strong>Results</strong>: Vindolinine was detected predominantly in the ethanolic extracts of leaves and stems, with retention times of 55.22 and 55.28 minutes, respectively, and was absent in flowers. The mass spectral data showed strong similarity to reference spectra (CAS: 5980-02-9), confirming its presence. The organ-specific pattern suggests higher biosynthetic accumulation in photosynthetically active tissues.<br /><strong>Conclusion</strong>: This study provides the first global report of vindolinine in V. herbacea, highlighting northern Iran as a natural source of this rare and pharmacologically crucial alkaloid. These findings expand the phytochemical knowledge of the species and provide a basis for future biotechnological, pharmacological, and conservation-oriented studies.https://immunoreg.shahed.ac.ir/article_4990_3a332650adfc09d0b25fccd57afcace2.pdf