The Association Between the rs1805329 of Rad23B Polymorphism and Severity of Lung Complications of Patients Exposed to Sulfur Mustard in Long Term

Document Type : Original Article

Authors
Mohammad Saber Zamani 1
Tooba Ghazanfari 2
Soghrat Faghihzadeh 3
1 Department of Biology, ّScience and Research Branch, Islamic Azad University, Tehran, Iran.
2 Immunoregulation Research Center, Shahed University, Tehran, Iran.
3 Department of Biostatistics and Social Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
10.32598/Immunoregulation.4.2.6
Abstract
Background: During the Iran-Iraq war, the Iraqi regime frequently used sulfur mustard gas as a weapon in various residential areas, including Sardasht, causing multiple and severe long-term destructive effects on all living organisms, including humans. The pulmonary system is among the most essential organs with varying severity involved in sulfur mustard chemotoxicity in the long term. This study aimed to evaluate the effects of Rad23B (rs1805329) polymorphism on the severity of lung complications in patients exposed to SM gas 30 years ago.
Materials and Methods: The study explored 106 severe and 105 mild PBMC patients exposed to SM gas. Furthermore, 47 PBMC samples were added to the study as a non-exposed group (control). Genotyping of rs1805329 was performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
Results: The results revealed no significant differences in genotype frequencies between the mild, severe, and the unexposed group. Moreover, no statistically significant difference was observed in genotypic frequency in all co-dominant, dominant, recessive, and over-dominant models.
Conclusion: The obtained data indicated that the rs1805329 of Rad23B was not associated with the severity of the lung complications of patients exposed to sulfur mustard 30 years ago.
Keywords
Mustard gas
DNA Repair
SNP
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Immunoregulation
Volume 4, Issue 2
Winter 2021
Pages 109-116