Effect of Aloe Vera Herbal Extract on Innate Immunity in Balb/c Mice Against Candida Albicans

Document Type : Original Article

Authors

1 Department of Medical Mycology, School of Medical Sciences, AJA University, Tehan, Iran

2 Immunoregulation Research Center, Shahed University, Tehran, Iran.

Abstract

Background: Based on the reports, Aloe vera extract, a herbal medicine, has antimicrobial and anti-inflammatory effects. Also, this medicinal plant has considerable effects on innate immunity through cytokine secretion which resist fungal infections such as Candida albicans. Moreover, Aloe vera is considered as immunomodulatory agent and regulate inflammatory responses.
Materials and Methods: In this study, twenty Balb/c mice were divided into five groups: four groups of infected mice with Candida albicans treated with different doses of Aloe vera extract and one healthy control group. Then, macrophage vital activity was measured by NBT assay. Nitric Oxide (NO) production as representative of macrophage activity was assayed by MTT test. Tumor Necrosis Factor alpha (TNF-α) and Interleukin-12 (IL-12) cytokines were measured by ELISA assay.
Results: Based on our study results, macrophage vital activity and NO production decreased significantly. However, TNF-α and IL-12 cytokines increased significantly in mice model infected by Candida albicans.
Conclusion: Aloe vera extract could enhance macrophage activity through increase in TNF-α and IL-12 cytokines in Candida albicans infections. Therefore, Aloe vera could stimulate innate immune responses to eliminate Candida albicans.

Keywords


Christaki EV. Florou-Paneri PCANDIDA Aloe vera: A plant for many uses. Journal of Food, Agriculture and Environment. 2010; 8(2):245-9.
Balan BJ, Niemcewicz M, Kocik J, Jung L, Skopinska-Rozewska E, Skopinski P. Oral administration of Aloe vera gel, anti-microbial and anti-inflammatory herbal remedy, stimulates cell-mediated immunity and antibody production in a mouse model. Central-European Journal of Immunology. 2014; 39(2):125-30. [DOI:10.5114/ceji.2014.43711] [PMID] [PMCID]
Goudarzi M, Fazeli M, Azad M, Seyedjavadi SS, Mousavi R. Aloe vera gel: Effective therapeutic agent against multidrug-resistant pseudomonas aeruginosa isolates recovered from burn wound infections. Chemotherapy Research and Practice. 2015; 2015:639806. [DOI:10.1155/2015/639806] [PMID] [PMCID]
Farahnejad Z, Ghazanfari T, Yaraee R. Immunomodulatory effects of Aloe vera and its fractions on response of macrophages against Candida albicans. Immunopharmacology and immunotoxicology. 2011; 33(4):676-81. [DOI:10.3109/08923973.2011.560158] [PMID]
Kuncharoen N, Muramatsu Y, Shibata C, Kamakura Y, Nakagawa Y, Tanasupawat S. Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f. International Journal of Systematic and Evolutionary Microbiology. 2017; 67(1):37-41. [DOI:10.1099/ijsem.0.001566] [PMID]
Kawakami K, Tohyama M, Xie Q, Saito A. IL‐12 protects mice against pulmonary and disseminated infection caused by Cryptococcus neoformans. Clinical & Experimental Immunology. 1996; 104(2):208-14. [DOI:10.1046/j.1365-2249.1996.14723.x] [PMCID]
Matsubara VH, Ishikawa KH, Ando-Suguimoto ES, Bueno-Silva B, Nakamae AEM, Mayer MPA. Probiotic bacteria alter pattern-recognition receptor expression and cytokine profile in a human macrophage model challenged with candida albicans and lipopolysaccharide. Frontiers in Microbiology. 2017; 8:2280. [DOI:10.3389/fmicb.2017.02280] [PMID] [PMCID]
Mehrad B, Strieter RM, Standiford TJ. Role of TNF-α in pulmonary host defense in murine invasive aspergillosis. The Journal of Immunology. 1999; 162(3):1633-40. [PMID]
Bernardes I, Felipe Rodrigues MP, Bacelli GK, Munin E, Alves LP, Costa MS. Aloe vera extract reduces both growth and germ tube formation by Candida albicans. Mycoses. 2012; 55(3):257-61. [DOI:10.1111/j.1439-0507.2011.02079.x] [PMID]
Zanuzzo FS, Sabioni RE, Montoya LNF, Favero G. Urbinati ECANDIDA Aloe vera enhances the innate immune response of pacu (Piaractus mesopotamicus) after transport stress and combined heat killed Aeromonas hydrophila infection. Fish & Shellfish Immunology. 2017; 65:198-205. [DOI:10.1016/j.fsi.2017.04.013] [PMID]
Farahnejad Z, Rasaee MJ, Moghadam MF, Paknejad M, Kashanian S, Rajabi M. Preparation and characterization of a monoclonal antibody against mannoprotein of Candida albicans. Hybridoma. 2005; 24(3):146-51. [DOI:10.1089/hyb.2005.24.146] [PMID]
Kriengkauykiat J, Ito JI, Dadwal SS. Epidemiology and treatment approaches in management of invasive fungal infections. Clinical Epidemiology. 2011; 3:175-91. [PMID] [PMCID]
Liu C, Leung MY, Koon JC, Zhu LF, Hui YZ, Yu B, et al. Macrophage activation by polysaccharide biological response modifier isolated from Aloe vera L. var. chinensis (Haw.) Berg. International Immunopharmacology. 2006; 6(11):1634-41. [DOI:10.1016/j.intimp.2006.04.013] [PMID]
Zhang L, Tizard IR. Activation of a mouse macrophage cell line by acemannan: The major carbohydrate fraction from Aloe vera gel. Immunopharmacology. 1996; 35(2):119-28. [DOI:10.1016/S0162-3109(96)00135-X]
Karaca K, Sharma JM, Nordgren R. Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera. International Journal of Immunopharmacology. 1995; 17(3):183-8. [DOI:10.1016/0192-0561(94)00102-T]
Mawarti H, Rajin M, Asumta Z. The effects of Aloe Vera on TNF-a levels, the percentage of Nk cells and Th 17 cells in rat that received izoniazid and rifampycin. Medical Archives. 2017; 71(5):308. [DOI:10.5455/medarh.2017.71.308-311] [PMID] [PMCID]
Habeeb F, Stables G, Bradbury F, Nong S, Cameron P, Plevin R, et al. The inner gel component of Aloe vera suppresses bacterial-induced pro-inflammatory cytokines from human immune cells. Methods. 2007; 42(4):388-93. [DOI:10.1016/j.ymeth.2007.03.005] [PMID]
Jovanovic DV, Di Battista JA, Martel-Pelletier J, Jolicoeur FC, He Y, Zhang M, et al. IL-17 stimulates the production and expression of proinflammatory cytokines, IL-β and TNF-α, by human macrophages. The Journal of Immunology. 1998; 160(7):3513-21. [PMID]
Munder M, Mallo M, Eichmann K, Modolell M. Murine macrophages secrete interferon γ upon combined stimulation with Interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation. Journal of Experimental Medicine. 1998; 187(12):2103-8. [DOI:10.1084/jem.187.12.2103] [PMID]
Goplen NP, Saxena V, Knudson KM, Schrum AG, Gil D, Daniels MA, et al. IL-12 signals through the TCR to support CD8 innate immune responses. The Journal of Immunology. 2016; 197(6):2434-43. [DOI:10.4049/jimmunol.1600037] [PMID] [PMCID]
Chen H, Li P, Yin Y, Cai X, Huang Z, Chen J, et al. The promotion of type 1 T helper cell responses to cationic polymers in vivo via toll-like receptor-4 mediated IL-12 secretion. Biomaterials. 2010; 31(32):8172-80. [DOI:10.1016/j.biomaterials.2010.07.056] [PMID]