Effect of Immune Responses Against Hydatid Cyst Antigens on Growth of Melanoma Tumor

Document Type : Original Article

Authors

1 Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.

2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

Background: Hydatid cyst is the larval stage of the tape worm parasite, Echinococcus granulosus. Human is infected by ingestion of parasite ova excreted in dog feces. The anticancer activity of different antigens of hydatid cyst has been reported in the previous works. In this research, the role of immune system in induction of this anticancer activity has been investigated.
Materials and Methods: Spleen cells of mice immunized with hydatid cyst antigens were transferred to the recipient mice that had already been challenged with melanoma cells. Also, mice with melanoma cancer were injected with antisera raised against hydatid cyst antigens. In all above mice, tumor growth was measured using a caliper.
Results: Passive transfer of spleen cells resulted in significant inhibition of melanoma cancer growth. However passive transfer of antisera did not affect cancer growth in the recipient mice.
Conclusion: According to the results of this work, cellular immunity but not humoral immunity may be involved in induction of anticancer activities of hydatid cyst.

Keywords


Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clinical Microbiology Reviews. 2004; 17(1):107-35. [DOI:10.1128/CMR.17.1.107-135.2004] [PMID] [PMCID]
Akgül H, Tez M, Ünal AE, Keşkek M, Sayek İ, Özçelik T. Echinococcus against cancer: Why not. Cancer. 2003; 98(9):1999-2000. [DOI:10.1002/cncr.11752] [PMID]
Berriel E, Russo S, Monin L, Festari MF, Berois N, Fernández G, et al. Antitumor activity of human hydatid cyst fluid in a murine model of colon cancer. The Scientific World Journal. 2013; 2013:230176. [DOI:10.1155/2013/230176]
Chookami MB, Sharafi SM, Sefiddashti RR, Bahadoran M, Pestechian N, Yousofi Darani H. [Effect of alive protoscoleces of hydatid cyst on the growth of melanoma cells in mouse model (Persian)]. Journal of Isfahan Medical School. 2014; 32(281):486-92.
Chookami MB, Sharafi SM, Sefiddashti RR, Jafari R, Bahadoran M, Pestechian N, et al. Effect of two hydatid cyst antigens on the growth of melanoma cancer in C57/black mice. Journal of Parasitic Diseases. 2016; 40(4):1170-3. [DOI:10.1007/s12639-015-0643-7] [PMID] [PMCID]
Zhang W, Li J, McManus DP. Concepts in immunology and diagnosis of hydatid disease. Clinical Microbiology Reviews. 2003; 16(1):18-36. [DOI:10.1128/CMR.16.1.18-36.2003] [PMID] [PMCID]
Yousofi Darani H, Soozangar N, Khorami S, Taji F, Yousofi M, Shirzad H. Hydatid cyst protoscolices induce cell death in WEHI-164 fibrosarcoma cells and inhibit the proliferation of baby hamster kidney fibroblasts in vitro. Journal of Parasitology Research. 2012; 2012: 304183. [DOI:10.1155/2012/304183]
Aref N, Shirzad H, Yousefi M, Yousofi Darani H. Effect of different hydatid cyst molecules on hela and vero cell lines growth in vitro. Journal of Immunodeficiency & Disorders. 2013; 2(1). [DOI:10.4172/2324-853X.1000105]
Darani HY, Yousefi M. Parasites and cancers: Parasite antigens as possible targets for cancer immunotherapy. Future Oncology. 2012; 8(12):1529-35. [DOI:10.2217/fon.12.155] [PMID]
Yuk JM, Shin DM, Song KS, Lim K, Kim KH, Lee SH, et al. Bacillus calmette-guerin cell wall cytoskeleton enhances colon cancer radiosensitivity through autophagy. Autophagy. 2010; 6(1):46-60. [DOI:10.4161/auto.6.1.10325]
Sabzehali F, Azimi H, Goudarzi M. Bacteria as a vehicle in cancer therapy and drug delivery. Journal of Paramedical Sciences. 2017; 8(1):52-9.
Dillman R. The history and rationale for monoclonal antibodies in the treatment of hematologic malignancy. Current Pharmaceutical Biotechnology. 2001; 2(4):293-300. [DOI:10.2174/1389201013378617] [PMID]
Finn OJ. Cancer immunology. New England Journal of Medicine. 2008; 358(25):2704-15. [DOI:10.1056/NEJMra072739] [PMID]
Errico DA, Medeiros A, Mıguez M, Casaravilla C, Malgor R, Carmona C, et al. O-glycosylation in Echinococcus granulosus: Identification and characterization of the carcinoma-associated Tn antigen. Experimental Parasitology. 2001; 98(2):100-9. [DOI:10.1006/expr.2001.4620] [PMID]
Thors C, Jansson B, Helin H, Linder E. Thomsen-Friedenreich oncofetal antigen in Schistosoma mansoni: Localization and immunogenicity in experimental mouse infection. Parasitology. 2006; 132(1):73-81. [DOI:10.1017/S003118200500867X] [PMID]
Ubillos L, Medeiros A, Cancela M, Casaravilla C, Salda-a J, Domínguez L, et al. Characterization of the carcinoma-associated Tk antigen in helminth parasites. Experimental Parasitology. 2007; 116(2):129-36. [DOI:10.1016/j.exppara.2006.12.009] [PMID]
Zenina A, Kravtsov E, Tsetsegsaikhan B, Yashina N, Dalin M, Karpenko L, et al. The study of immunological component in antitumor effect of Trypanosoma cruzi. Bulletin of Experimental Biology and Medicine. 2008; 145(3):352-4. [DOI:10.1007/s10517-008-0089-3] [PMID]
Zhigunova A, Kravtsov E, Yashina N, Dalin M, Karpenko L. Effects of specific antibodies and immunocompetent cells on tumor growth in passive transfer experiment. Bulletin of Experimental Biology and Medicine. 2013; 154(6):762-4. [DOI:10.1007/s10517-013-2050-3] [PMID]
Woodruff M, Nolan B. Preliminary observations on treatment of advanced cancer by injection of allogeneic spleen cells. The Lancet. 1963; 282(7305):426-9. [DOI:10.1016/S0140-6736(63)92171-8]
Amri M, Mezioug D, Ait-Aissa S, Touil-Boukoffa C. In vivo IL-12 and IL-8 production in hydatic patients and their possible diffusion in hydatid cysts. European Cytokine NetWork. 2008; 19(3):142-8. [PMID]
Emery I, Leclerc C, Sengphommachanh K, Vuitton DA, Liance M. In vivo treatment with recombinant IL‐12 protects C57BL/6J mice against secondary alveolar echinococcosis. Parasite Immunology. 1998; 20(2):81-91. [DOI:10.1046/j.1365-3024.1998.00131.x] [PMID]
Karadayi S, Arslan S, Sumer Z, Turan M, Sumer H, Karadayi K. Does hydatid disease have protective effects against lung cancer. Molecular Biology Reports. 2013; 40(8):4701-4. [DOI:10.1007/s11033-013-2565-8] [PMID]
Akdis M, Burgler S, Crameri R, Eiwegger T, Fujita H, Gomez E, et al. Interleukins, from 1 to 37, and interferon-γ: receptors, functions, and roles in diseases. Journal of Allergy and Clinical Immunology. 2011; 127(3):701-21. [DOI:10.1016/j.jaci.2010.11.050]
Carter P, Presta L, Gorman CM, Ridgway J, Henner D, Wong W, et al. Humanization of an anti-p185HER2 antibody for human cancer therapy. Proceedings of the National Academy of Sciences. 1992; 89(10):4285-9. [DOI:10.1073/pnas.89.10.4285]
Kallinikova VD, Batmonkh TS, Kosobokova EN, Pakhorukova LV, Ogloblina TA, Kravtsov EG, et al. Antibodies against Trypanosoma cruzi in intact mice and their oncoprotective effect. Meditsinskaia Parazitologiia i Parazitarnye Bolezni. 2008; 1(1):11-5.